Previously, I discussed the circumstances that
might persuade defense attorneys to explore using a DNA expert. One of
these perplexing circumstances is driven—in part—by astonishing
advances in the sensitivity of DNA detection. Recent DNA technologies,
referred to as
LOW COPY NUMBER (LCN) analysis (also called ‘low template’, ‘high sensitivity’, or ‘trace DNA’ analysis) allows crime lab analysts to
PUSH DNA DETECTION CAPABILITIES
to the point at which useful data is obtained from only 15-20 cells,
or perhaps as few as just 1 or 2 cells. Such extremes in testing
sensitivity are causing courtroom battles to emerge, due to the mere
LIMITATIONS OF SCIENTIFIC ACCEPTANCE.
Adding fuel to this fire, these conflicts intensify significantly when
DNA expert witnesses fall into the trap I prefer to call the
'touch DNA misnomer'.
Embracing the phrase "touch DNA" on
the witness stand, with NO scientific proof that touching ever
occurred, is the 2011-2012 idiotic equivalent of yesteryear's 'DNA Fingerprinting misnomer'.
STR-based forensic DNA typing technology involves NO examination of
fingers. Nor does it relate to latent print examinations, an
entirely separate forensic discipline. Why do some individuals
insist upon confusing society with terms that simply do not apply?
HOW DOES ANY TYPE OF TRACE MATERIAL END UP ON A SPECIFIC SURFACE?
In 1910, Dr. Edmond Locard, professor of forensic medicine at the
University of Lyons, France, was the founder of the world's first
forensic science lab. This great scientist was also the first
forensic scientist to formally emphasize the importance of transfer
events in the investigation of crimes.
Dr. Locard's incredible intuition evolved into the time-tested
Locard Exchange Principal, stating that
"Every contact leaves a trace."
Locard's principal became universally accepted over forty years
BEFORE James Watson and Francis Crick proposed the first accurate model
of the DNA double helix. The exchange principal was also widely
embraced over seventy years BEFORE Colin Pitchfork became the first
person to be identified and convicted of a crime, using the power of DNA
typing.
As an insult to the celebrated history of
Dr. Locard's principal, *some* modern day prosecutors—with the support
of their crime lab analysts—attempt to downplay the plausibility of
DNA transfer events. Despite the fact that the crime scenes are
crawling with CSI's who enthusiastically center their efforts on
Locard's teachings, some courtroom comedians are allowed to drone on
with their ridiculous folly of reasoning. JURORS PLEASE TAKE NOTE: TRACE MATERIAL EXCHANGE EVENTS ARE NOT THE PRODUCT OF OVERACTIVE SCIENTIFIC IMAGINATIONS.
The ludicrous efforts to downplay Locard are
readily invalidated. EVERY WINTER—new strains of common cold viruses
and influenza viruses succeed with their inevitable campaigns of terror
throughout earth's human population. How do these prosecutors and
their supporting scientists (and I am using the term ‘scientists’
loosely) suppose these viruses are so swiftly spread from human to
human?
During a particularly frosty stretch of
weather, look around, visit various homes, take a stroll through the
local mall. Nasal cavities are draining, infected individuals are
coughing and sneezing. Crumpled up facial tissues are strewn
EVERYWHERE. Those tissues came from the people who are actually
considerate enough to use a Kleenex every now and then—rather than
their shirt sleeves OR THEIR HANDS.
Trillions of viral particles are spread by
the actions of the infected, ….to door handles, telephones, computer
keyboards, car keys, steering wheels, stairway railings, currency,
vending machines, TV remote controls, pens, pencils, clothing, bedding,
the list is endless. Each year, we are BEGGED by the Centers for
Disease Control to “Wash your hands!” Any healthy person, who fails to
wash his hands, and makes the mistake of rubbing his own tired eyes,
….well, ….you do the math. It takes a number of days for viral
particles to establish a foothold in a human respiratory system.
DNA differs very little from viruses. Yes,
our genetic molecules are much more friendly, and not very invasive.
DNA causes no sore throats, no runny noses, and no coughs. Beyond that,
DNA and viruses are quite similar in that they are both
submicroscopic clumps of matter. Transfer events DO occur with BOTH
forms of matter. Please do not let any courtroom snake oil
representatives succeed in convincing you otherwise.
Today's state-of-the-art DNA detection technology can decipher a full DNA profile from less than one BILLIONTH
of a gram of DNA. One way to grasp such extraordinarily tiny amounts
of DNA is to visualize the mass of material in a standard packet of
artificial sweetener. These packets contain one gram of
material. Imagine setting aside 1/1000th of a single packet and
disposing of the remaining 999 parts. The spec of powder set aside
would weigh one milligram. Now imagine setting aside 1/1000th of this milligram and discarding the remainder. You now have one microgram
of material (which is 1/1 millionth of the original sweetener
packet). This amount of material cannot be clearly seen without the
use of a microscope. By some means, you must now set aside 1/1000th of
your microgram of artificial sweetener-this is one nanogram,
or 1/1 BILLIONTH of the original starting material. One nanogram of DNA
is PLENTY of genetic material for generating a FULL DNA profile. The
astonishing sensitivity of forensic DNA typing technology does not
diminish the fact that we are indeed working with a profoundly tiny
mass of DNA.
Scientific debates focusing on the mechanisms
of casual/incidental transfer events, involving such tiny amounts of
DNA, are far from settled. In December 2010, some of the world's most
renowned authorities on forensic trace DNA (Roland Oorschot, Kaye
Ballantyne, and R. John Mitchell), published a
REVIEW in
Investigative Genetics. Quoting these authors directly from the
"Transfer Issues" section of the review:
"Greater effort needs to be made by
police/crime investigators to investigate how a DNA sample arrived at
the location where it was found, as well as by scientists to better
understand the impact of activities on the relative amounts of DNA from
particular sources at a crime scene. In some instances, it is
possible to derive the chain of events that led to a trace DNA sample
being present at a crime scene - for example, prior visits to the scene
or the known use of an item. Awareness of these variables, and their
impact on transfer events, will assist in weighting the likelihood of
proposed alternative scenarios."
The following is a quote from this article:
"The examination of evidence for handler
DNA can reveal DNA of people who have, or have not, handled the item;
the stronger profile may, or may not, be the person who last handled
the item; An inference of direct contact between an individual and the
item may or may not be supportable, depending on the circumstances of
the case. In other words, we did not know enough to make any sensible
scientific judgements as to how DNA came to be on an item."
Later, the article continues:
"Frequently, the underlying hypothesis is
that touching, or direct contact, is a more likely scientific
explanation for the finding of a DNA profile on an item than indirect
contact. This to the extent that it may be described as providing
‘extremely strong’ support for direct versus indirect transfer. In our
view, such an opinion on DNA transfer is not supportable based on
case experience or on the available scientific research."
"Obviously, the inadvertent transfer of
DNA is an area that should be further studied. Since so many of the
available journal articles present conflicting information, more work
is needed to see how likely it is to both transfer and detect DNA in a
secondary or even a tertiary fashion, especially considering the
sensitivity of modern forensic DNA analysis."
It is vital to keep in mind that the average
adult human sheds approximately 36,000 skin cells every 60 seconds.
This number varies broadly among individuals, as there are profound
differences between those who can be characterized as 'good shedders', and others who are 'poor shedders'.
A single drop of saliva, expelled during a
cough or a sneeze, will contain approximately 500,000 salivary
epithelial cells. Forensic Biologists can attest to the fact that 500
to 10,000 nanograms of DNA are routinely recovered during collection of a
single oral swab. Once again, recall that ONLY ONE NANOGRAM is
optimal for generating a complete DNA profile. This mass of DNA can be
readily extracted from as few as 200 cells. This tiny number of cells
could sit-nearly invisible-upon the very tip of a toothpick.
How many falsely accused individuals have
been wrongfully imprisoned as a consequence of a few hundred cells
finding their way to an incriminating location?
Michael J. Spence, Ph.D.
March 7, 2012